Ethanolic Extraction, Phytochemical Screening, Antimicrobial and Antioxidant activities of Chamaecyparis obtuse Fruits .

Abstract

This study was carried out in Khartoum State-Sudan, during October (2019). The species Chamaecyparis obtusa (Crippsii) belong to familyCupressaceae , locally known as (Shagarat Bakhor Alanfar) was chosen because it’s using traditionally in treatment of many abdominal disease. Phytochemical activities were investigated to detect the effects of antimicrobial and antioxidant; this plant was collected from Gabal maraha , South Darfur State, western Sudan. The dried fruits of Chamaecyparis Obtusawas extracted successively with (ethanol), The phytochemical screening carried out on ethanolic extract of species fruits and showed as table: (1) , that there were secondary metabolize ( alkaloids ,flavonoids, sterol, tannins , triturpenes ,cardiac glycoside, phenol and absent of saponnins). The antimicrobial activity of extract were evaluated against two standard bacteria (Gram positive; Staphylococcus aureus) and (Gram negative; Escherichia coli,), the results of antimicrobial activities of extract at different concentrations and inhibition zone were measured in (mm) table (2) the range of inhibition was found between 15-22mm .Antioxidant was tested and The result of it cited that there is high antioxidant activity in the extraction table (3).